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Agonist-induced FPR1 internalization is not dependent on Gα-mediated signal transmission. (a) Cells were pretreated either for 16 h with PTX or (b) for 1 h with FR and subsequently stimulated with agonist concentrations eliciting the respective Emax. Results were normalized to the 10−4 M W-peptide-induced maximum response. Data points represent the mean ± SEM of 6 independent measurements.
