Figure 1.

(A) Normal human intestine was stained in indirect immunohistochemistry on FFPE (formalin-fixed, paraffin embedded) serial sections for RORC, clone 6F3.1 as per Gendusa et al.³ and in indirect immunofluorescence (inset; green) together with CD3 (clone CD3-12; red) as per Bolognesi et al.² Nuclear counterstain is hematoxylin and 4′,6-diamidino-2-phenylindole (DAPI; blue) respectively. On the left, no stripping occurred before staining. On the right, sections were stripped for 10×-equivalent (5 hr) or 4×-equivalent (2 hr; inset) before staining. and (B) normal human FFPE tonsil was stained for GATA3, goat antibody AF2605-SP (green) in indirect immunofluorescence as per Bolognesi et al.² as the 12th cycle of staining and stripping. Nuclear counterstain DAPI (blue), no autofluorescence was digitally subtracted. Bars A and B = 100 µm