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. 2020 Mar 26;9(4):797. doi: 10.3390/cells9040797

Figure 1.

Figure 1

Intravenous transplantation of prostate cancer (PCa) cells induces extravasation and perivascular metastasis formation. (a) Schematic indication of cancer cell engraftment. PC-3M-Pro4-mCherry was injected into zebrafish (ZF) vasculature. Metastases was formed at 6 dpi. Red, cancer cells. Green, vessels. (b–c) High-resolution images were acquired using a Light-sheet microscope. Scale bar=100 um. (b) Three-dimensional overview of metastatic phenotype of the cancer cells at 6 dpi. Left, lateral. Middle, posterior. Right, Ventral. Up, solid vessels. Down, transparent vessels. Imaging was acquired using Zeiss Lightsheet Z1 at 40x magnification. Zeiss ZEN software was used for multiview fusion and deconvolution. Image J and Imaris 8.1 were applied for imaging stitching and 3D rendering. (c) High-magnification images to show single cell behaviors. Single cells were extravasated from intersegmental vessel (ISV), dorsal longitudinal anastomotic vessel (DLV) and Dorsal vein (DA). Up, solid vessels. Down, transparent vessels. (d–e) Whole-mount immunofluorescence against phosphorylated Histone 3 (d) and Ki-67 (e) at 6 dpi. Images were acquired using confocal. Scale bar = 50um.