Skip to main content
. 2020 Mar 26;9(4):797. doi: 10.3390/cells9040797

Figure 5.

Figure 5

Cripto drives the metastatic phenotype through controlling EMT plasticity. (a) PC-3M-Pro4-mCherry-SCR and PC-3M-Pro4-mCherry Cripto kd were injected into ZF. Confocal images were acquired at 6 dpi. Green, vessels. Red, cancer cells. Scale bar = 50um. Cancer cell burden at the metastatic site was quantified by measuring total fluorescence intensity at 6 dpi. Group size = 30. (b) Expression of Cripto, stemness markers and EMT markers was measured in PC-3M-Pro4-mCherry-SCR and PC-3M-Pro4-mCherry-Cripto kd in culture and in ZF metastases at 6 dpi. Experiments were independently repeated three times. Data were presented as mean ± SD (c) Expression of EMT markers was compared between PC-3M-Pro4-mCherry-SCR, -Snail1 kd and -Zeb1 kd. Experiments were independently repeated three times. Data were presented as mean ± SD (d) PC-3M-Pro4-mCherry-SCR, -Snail1 kd and -Zeb1 kd were injected into ZF. Confocal images were acquired at 1, 4 and 6 dpi. Green, vessels. Red, cancer cells. Scale bar = 50um. (e) Cancer cell burden at the metastatic site was quantified by measuring total fluorescence intensity. Group size = 30. Data were presented as mean ± SEM.