(A) Chromosomal location of the Sarm1 gene, chemokine locus, and Xaf1 gene (UCSC genome browser).
(B) Sequence analysis of SNPs in the Ccl5 gene of WT and Sarm1AD mice.
(C) WT and Sarm1AD macrophages were stimulated with 10 ng/mL of LPS for 3 h. Cytokine production was measured by qPCR.
(D) WT, Sarm1+/AD, and Sarm1AD/AD macrophages were stimulated as in (C) (the bottom graph shows the same data as the top graph on a different scale).
(C) and (D) show mean ± SD for triplicate biological replicates and are representative of 3 experiments. *p < 0.05 and **p < 0.01 (unpaired t test); ns, not significant.