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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: Cell Rep. 2020 Apr 7;31(1):107498. doi: 10.1016/j.celrep.2020.03.062

Figure 3. SARM1 Knockdown and Overexpression Do Not Modulate Chemokine Production.

Figure 3.

(A) RAW-V5 cells were treated with Sarm1 siRNAs. Sarm1 knockdown efficiency was measured by qPCR (left), or Ccl4 expression was measured after treatment with 10 ng/mL of LPS for 3 h (right).

(B) RAW-SARM1-V5 cells treated as in (A).

(C) Western blot of SARM1 expression in RAW-SARM1-V5 cells treated with Sarm1 siRNAs.

(D) Ccl4 expression by qPCR after treatment with 10 ng/mL of LPS for 3 h in WT and Sarm1AD macrophages after Sarm1 siRNA knockdown.

(E) RAW-V5 and RAW-SARM1-V5 cells were treated with 10 ng/mL of LPS. Cytokine production was measured at 3 h by qPCR.

Graphs show mean ± SD of triplicate biological samples and are representative of 3 experiments. **p < 0.01 (unpaired t test). See full gel image in Figure S1.