KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| IκBα | Cell Signaling | Cat#9242; RRIF:AB_331623 |
| rabbit phosphor-SAPK/JNK (Thr183/Tyr185) | Cell Signaling | Cat#9251; RRID:AB_331659 |
| rabbit phosphor-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | Cell Signaling | Cat# 4370; RRID:AB_2315112 |
| rabbit phosphor-p38 MAPK (Thr180/Tyr182) | Cell Signaling | Cat#9211; RRID:AB_331641 |
| mouse phosphor-Akt (Ser 473) (587F11) | Cell Signaling | Cat# 4051; RRID:AB_331158 |
| rabbit Xaf1 (aa166-194) | LS Bio | Cat#LS-C158287 |
| rabbit β-Actin HRP | Cell Signaling | Cat#5125; RRID:AB_1903890 |
| mouse V5-HRP | Serotec | Cat#MCA1360P |
| Bacterial and Virus Strains | ||
| NDV-GFP | Adolfo García-Sastre (Park et al., 2003) | N/A |
| VSV Indiana | Adolfo García-Sastre | N/A |
| West Nile virus-NY99 | Jean Lim | N/A |
| LACV original (parent) strain | Andrew Pekosz | N/A |
| WNV-Kunjin | Jean Lim | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| rmM-CSF | R&D Systems | Cat#416-ML |
| Poly(I:C) HMW | Invivogen | Cat#tlrl-pic |
| E.coli 0111:B4 LPS purified by gel filtration | Sigma | Sigma L3012 |
| R848 | Invivogen | Cat#tlrl-r848 |
| CL075 | Invivogen | Cat#tlrl-c75 |
| TNF-α | R&D Systems | Cat#410-MT-010 |
| universal type I IFN | PBL Interferon | Cat#11200-2 |
| Fura-2-AM | Invitrogen | Cat#F-1221 |
| Critical Commercial Assays | ||
| Surveyor Mutation Detection Kit | IDT | Cat#706020 |
| EZNA total RNA kit | Omega | Cat#R6834-02 |
| RNase-free DNase | Omega | Cat#E1091 |
| Maxima Reverse Transcriptase and oligo-dT | Thermo | Cat#EP0743 |
| LightCycler 480 SYBR Green I Master Mix | Roche | Cat#04887352001 |
| Verikine Mouse IFN Alpha ELISA Kit | PBL interferon | Cat#42400-1 |
| TNF ELISA kit | BD biosciences | Cat#558534 |
| mouse CCL3/MIP-1α DuoSet | R&D systems | Cat#DY450 |
| TruSeq Stranded Total RNA Sample Preparation kit | Illumina | Cat#20020596 |
| Accell siRNA targeting Sarm1(1) | Horizon | Cat#A-041633-13-0020 |
| Accell siRNA targeting Sarm1(3) | Horizon | Cat#A-041633-15-0020 |
| Deposited Data | ||
| Uninfected and WNV infected C57BL6/J and Sarm1em3AGS brainstem gene expression | This paper | GEO: GSE136221 |
| RNaseq of C57BL/6J and C57BL6/J Sarm1AD brain to determine genetic background | This paper | GEO: GSE136284 |
| 129 Xaf1 sequence variants | This paper | GenBank: MN366017-366019 |
| Experimental Models: Cell Lines | ||
| RAW-V5 | This paper | N/A |
| RAW-SARM1-V5 | This paper | N/A |
| 3T3-Xaf1−/− | This paper | N/A |
| Sarm1MSD MEFs | This paper | N/A |
| Neuro2A | This paper | N/A |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J | Jackson | Cat#000664 |
| Sarm1AD | (Kim et al., 2007) | N/A |
| Sarm1AGS3 | This paper, deposited with Jackson Labs | Cat#034399 |
| Sarm1AGS12 | This paper, available upon request | N/A |
| Oligonucleotides | ||
| See Table S4 | N/A | |
| Recombinant DNA | ||
| pSpCas9(BB)-2A-GFP | (Ran et al., 2013) | Addgene#48138 |
| pSpCas9n(BB)-2A-GFP | (Ran et al., 2013) | Addgene#48140 |
| pLVX-IRES-Puro lentiviral vector | Takara | Cat#632183 |
| Software and Algorithms | ||
| cutadapt | (Martin, 2011) | https://doi.org/10.14806/ej.17.1.200 |
| STAR (v2.5.3a) | (Dobin et al., 2013) | N/A |
| STRING database | (Szklarczyk et al., 2017) | N/A |