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. 2020 Apr 2;9(4):859. doi: 10.3390/cells9040859

Figure 1.

Figure 1

Expression of miR-154-3p and miR-154-5p during development and after hyperoxic lung injury: A. Expression of miR-154-3p in the lung from E10.5- adult age. B. FISH of miR-154-3p expression at E17.5 and P2. C. miR-154-3p expression in hyperoxic lung injury at P2, P5 and P8 by FISH and Quantification of miR-154-3p positive cells in bronchiolar and alveolar epithelium. D. miR-154-3p and miR-154-5p expression in whole lung and isolated AT2 cells by RT-qPCR. Please note: for each stage the expression of miR-154-3p and -5p in the hyperoxia have been normalized to the expression in normoxia. P2: NOX n = 2, HOX n = 3; P5: NOX n = 3, HOX n = 4; P8: NOX n = 3, HOX n = 3; P3: NOX n = 6, HOX n = 6. Scale bar in B: low magnification: 120 μm; high magnification: 30 μm. Scale bar C: low magnification: 180 μm; high magnification: 45 μm. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.