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. 2020 Apr 2;9(4):859. doi: 10.3390/cells9040859

Figure 2.

Figure 2

Generation/validation of transgenic mice for miR-154 overexpression and pull-down assay. A. Scheme of miR-154 transgene and genotyping results from Tg(tet(O)miR-154) mice. B. Scheme of Snx19 gene and the Tg integration site. Expression of Snx19 after Tg integration was analyzed by qPCR in embryonic lungs. C+D. Increased expression of miR-154-3p and miR-154-5p in double transgenic mice [Tg((Scgb1a1-rtTA)/+; Tg(miR-154)/+] versus WT [Tg((Scgb1a1-rtTA)/+; +/+] after Dox induction from E18 to P16. E–G. Pull down biotinylated miR-154 versus gain of function with mimic in MLE12 cells. [Rosa26rtTa/rtTA; Tg(miR-154)/+] experimental and [Rosa26rtTa/rtTA; +/+] control embryos exposed to Dox in utero from E7.5 to E18.5 were generated and the lungs collected for genearray analysis to identify the genes downregulated upon miR-154 overexpression. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.