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. 2020 Mar 29;9(4):824. doi: 10.3390/cells9040824

Figure 3.

Figure 3

Loss of hyaluronan in ECs decreases angiopoietin 1 presence in ECs. (A) Representative images of angiopoietin 1 (Ang1, top) staining and BS1-lectin staining (middle) for ECs localization (scale bar = 20 µm) and (B) quantification of endothelial associated Ang1 in ligated and non-ligated gastrocnemius muscle of Has2-cKO (n = 5) and control (n = 3) mice. (C) Representative overlay images of platelet-derived growth factor receptor beta (PDGFRβ, green), laminin (red) and co-staining (yellow) in ligated and non-ligated gastrocnemius muscle of Has2-cKO (n = 5) and control (n = 3) mice (scale bar = 20 µm). (D) Representative overlay images of vascular endothelial growth factor 165 (VEGF165, green), BS1-lectin (red) and (E) quantification of VEGF165 and EC area ratio in ligated and non-ligated gastrocnemius muscle of Has2-cKO (n = 5) and control (n = 3) mice (scale bar = 20 µm). Values are given as mean ± SD. * p < 0.05.