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. 2020 Mar 27;9(4):816. doi: 10.3390/cells9040816

Figure 1.

Figure 1

Modulation of yes-associated protein (YAP) localization in wild-type (WT) and mutant muscle stem cells (MuSCs). (A) Confocal images of YAP (green) in WT and LMNAΔK32 mutant MuSCs cultured in low and high density conditions. Nuclei are stained with Hoechst (blue). Scale bar: 20 µm. (B) Quantification of YAP nucleo-cytoplasmic (N/C) ratio in WT, LMNAΔK32, LMNAL380S, LMNAR249W, and nesprin-1 KASK mutant MuSCs. Pooled values of WT (WT1 and WT2) are presented. Values are expressed as mean ± SEM, n = 200 cells for each cell line. * p < 0.05 vs WT; $ < 0.05 vs. corresponding sparse condition. (C) Micro-patterning modulation of YAP. Confocal images of YAP (green) in WT and LMNAΔK32 cells cultured on small ECM substrate that limits cell spreading. Nuclei are stained with Hoechst (blue). Scale bar: 30 µm. (D) Quantification of YAP nucleo-cytoplasmic (N/C) ratio on small ECM substrates. Values are expressed as mean ± SEM, n ≥ 62 cells for each cell line. * p < 0.05 vs. WT.