Figure 6.

Cytokine engineered MSCs modulate a cytolytic CAR T cell attack. Anti-CEA CAR T cells and non-modified T cells for control (each 1.5 × 10⁴ cells/well) were co-cultivated for 48 h with CEA⁺ LS174T or CEA⁻ Colo320 tumor cells (each 2.5 × 10⁴ cells/well) and non-modified or IL7 and IL7/IL12 secreting MSCs (each 3 × 10³ cells/well) in 96-well round bottom plates. Viability of tumor cells was determined by a tetrazolium salt based XTT-assay. Cytolysis [%] was determined by recording the reduction in viability. Values represent the mean of replicates +/− standard deviation (SD). Significant differences were calculated by Student’s T test, * p < 0.05.