Figure 2.

Relative expression changes in miR-29b (A) and Col1α1 (B) in cultured cardiac fibroblasts isolated from male and female myocardium, stimulated with TGF-β1 (10 ng/mL) for 24 h in serum-free medium. Relative changes in the expression of miR-29b in cardiac fibroblasts from male and female mice stimulated with 17β-estradiol (5 and 10 nM) for 24 h (C). Data are reported as mean ± SEM * p < 0.05, *** p < 0.001 (two way ANOVA and Bonferroni’s post-hoc test).