Figure 3.

Donor-to-donor variation in the generation of CD56⁺ NK cells from mobilized PB-CD34⁺ cells. Mobilized HSCs from seven healthy donors were cultured ex vivo following the scheme depicted in Figure 1A. (A) Development of CD56⁺ NK cells was analyzed at the indicated time points of the culture period by flow cytometry. For donors 1 and 2, no data were available for day 56, or for days 35 and 56, respectively. (B) Cytotoxicity of CD56⁺ NK cells generated from PB-CD34⁺ cells was determined in flow-cytometry-based cell killing assays at an effector to target (E/T) ratio of 10:1. Mean values ± SEM of three technical replicates for each donor are shown. Identical donor numbers in (A) and (B) indicate the same donors.