Figure 1.
Effects of nobiletin (NOB) on the cell growth of K562 cells. (a) The chemical structure of nobiletin (5,6,7,8,3′,4′-hexamethoxyflavone, NOB). (b) K562 cells were treated with vehicle (0.1% DMSO) or NOB (10-100 μM) for 24 h or 48 h. Cell viability was measured using an MTT assay. (c) Cells at an initial seeding concentration of 2 × 105/mL were incubated with NOB (10-100 μM) for 24 h or 48 h. The cell numbers were measured by counting of viable cells using trypan blue staining. The experiments were repeated three times. These data represent the mean ± SD of three independent experiments. *p < 0.05 and **p < 0.01 represent significant differences compared with the vehicle-treated cells (veh).