Skip to main content
. 2020 Apr 4;9(4):886. doi: 10.3390/cells9040886

Figure 2.

Figure 2

Depletion of SPHK1 inhibits HCC38 CSC and non-CSC proliferation. (A,B) Efficient depletion of SPHK1 expression was achieved using two independent lentiviral shRNA constructs in non-CSC and CSC cultures. Vector (Vec) and non-silencing (NS) controls were included for accurate assessments of knockdown efficiency; GAPDH was used as a loading control. (C,D) Morphological changes were observed following knockdown of SPHK1 in non-CSC and CSC cultures. (E,F) Cell proliferation between 0 and 72 h was significantly reduced in SPHK1-depleted non-CSC and CSC cultures. Points represent the means ± s.d. of three independent experiments. Asterisks (*) indicate statistical significance compared with non-silencing (NS) control cells (p < 0.01, Student’s t-test).