Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 4;9(4):886. doi: 10.3390/cells9040886

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Multidimensional protein identification technology (MudPIT) identifies novel targets of SPHK1. (A) SPHK1 was overexpressed in HEK-293 cells. Protein lysates were isolated and subjected to MudPIT analyses. As evident from the FLAG-tag immunoblot, virtually all of the detected SPHK1 is exogenously expressed. (B) A total of 34 proteins were upregulated (more than two-fold,) while 74 proteins were downregulated. (C) The top 10 most enriched GO terms in proteins deregulated by SPHK1 overexpression, determined by DAVID enrichment analysis. Type I and II interferon signaling are two of the most significantly perturbed pathways in SPHK1-overexpressing cells. (D) Protein-protein interaction network of SPHK1 target proteins. Networks were generated with STRING using a confidence threshold of 0.7.