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. 2020 Apr 1;9(4):854. doi: 10.3390/cells9040854

Figure 6.

Figure 6

Nef-induced senescence was associated with impaired adipogenesis. After 30 days of treatment with Tat or Nef, ASCs, isolated from different abdominal SCAT healthy donors, were induced to differentiate into adipocytes for 14 days. The Tat or Nef treatment was stopped during differentiation. (A) Adipogenic potential of ASCs was determined using Oil-Red-O staining 14 days after the start of differentiation induction. Representative pictures of cultured cells are shown (left panel), and the intensity of Oil-Red-O staining was normalized against protein content and expressed as the mean ± SEM % of control cells (n = 4, in duplicate). (B) The relative mRNA expression levels of PPARG, CEBPA, and FABP4 were normalized against that of PPIA. The results are expressed as the mean ± SEM % of control cells (n = 4, in duplicate). (C) Secreted leptin and adiponectin levels in the culture medium from the last 24 h of incubation were determined using ELISAs (n = 3, in duplicate). The results are expressed as mean ± SEM. * P < 0.05, ** P <0.01, **** P < 0.0001 vs. control cells.