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Kinetics of HspBP1 recruitment to stress granules. HeLa cells were treated with vehicle (control) or exposed to DEM. After a 4-hour DEM treatment, cells were transferred to medium without DEM for recovery (bottom panels). Samples were fixed at the time point indicated, and the distribution of HspBP1 and HuR was examined by immunocytochemistry (Materials and Methods). HspBP1 remained associated with SGs during the recovery from stress. DAPI demarcated nuclei; scale bar is 20 μm.
