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. 2020 Mar 29;9(4):825. doi: 10.3390/cells9040825

Figure 6.

Figure 6

HspBP1 knockdown impairs SG formation. HeLa cells were incubated for 4 days with the transfection agent lipofectamine, mock-treated, transfected with a control plasmid (scrambled) or constructs targeting two different regions of the HspBP1 transcript (shRNA1 and shRNA2). (A) Crude cell extracts were probed for HspBP1; actin was used as loading control. Quantitative Western blotting revealed a significant depletion of HspBP1 for each of the sh-constructs. All lanes were on the same blot. Results are shown as average +SEM. Significant differences were identified with One-Way ANOVA combined with Bonferroni posthoc analysis; * p < 0.05. (B) HeLa cells were transfected with control DNA or individual HspBP1 knockdown plasmids and subsequently treated with DEM. SGs were identified with HuR. Scale bar is 20 μm. (C) Using HuR as a marker, the SG area/cell and number of SGs/cell were quantified for control and HspBP1 knockdown cells. Bar graphs depict averages +SEM. Significant differences were determined with One-Way ANOVA and Bonferroni correction, control cells served as the reference; scram., scrambled sequence. * p < 0.05.