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. 2020 Mar 29;9(4):825. doi: 10.3390/cells9040825

Figure 7.

Figure 7

Production of HspBP1 full length protein and deletion mutants. (A) Organization of the HspBP1 protein. HspBP1 domains and the regions interacting with hsc70 are shown for the full length protein [16]. The organization of deletion mutants is also depicted. (B) Western blot analysis of full length and mutant HspBP1. Crude extracts were prepared for transfected HeLa cells and probed with antibodies against HspBP1 or hsp72. Actin was used as loading control. Lipo, lipofectamine; ctl, control plasmid; full length HspBP1, ΔM (deletion of residues 154–195), ΔMC (deletion of residues 154–195 and 314–359) ΔC (deletion of residues 314–359). All lanes were on the same blot.