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. 2020 Apr 10;9(4):921. doi: 10.3390/cells9040921

Figure 1.

Figure 1

(A) Real-time PCR analyses for formyl peptide receptor-2 (FPR2) mRNA relative to 18S rRNA. FPR2 mRNA relative to 18S rRNA in SGA (n = 28) and control (n = 52) in early gestation was determined using real-time PCR. Gene expression differences between SGA and control placental tissues were calculated according to 2-ΔΔCT method (Livak and Schmittgen, 2001). Data presented as mean ± SEM. A probability value of <0.05 was considered to be statistically significant as denoted by *. (B) ELISA. FPR2 concentrations in the chorionic villus tissues from SGA (n = 10) and control (n = 10) pregnancies. Data presented as mean ± SEM. A probability value of <0.05 was considered to be statistically significant as denoted by *. (C) Immunohistochemical localization for FPR2 protein. Immunoreactive FPR2 protein localization in placental tissues obtained from pregnancies at first trimester (n = 3) was performed using immunohistochemistry. A representative image is shown. Arrows indicate the presence of immunoreactive FPR2 protein in; extravillous cytotrophoblasts (EVCT), in the decidual cells and in the glandular epithelial cells. No immunoreactivity was observed in the negative control, where the primary antibody was substituted with the isotype control IgG2a (Figure 1Cii).