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. 2020 Mar 31;9(4):840. doi: 10.3390/cells9040840

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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OVOL2 interacts with the Id2 gene. (A) ChIP was performed using OVOL2 or IgG (negative control) antibodies in lysates of mouse TS cells differentiated for 3 days. Quantitative PCR was used to detect enrichment, using primers spanning CCGTTA sequences near transcription start sites of Id2, Eomes, and Esrrb. (B) Relative luciferase activity measured in HEK-293T cells transfected with Id2-Luc and either the CTRL plasmid or pEF-OVOL2-FLAG. (C) Quantitative RT-PCR showing relative expression of Cdx2, Eomes, Esrrb, and Id2 in mouse TS cells transfected with CTRL or pEF-ID2-FLAG (ID2 OE). Values significantly different (N = 3, P < 0.05) from controls are indicated with an asterisk (*). Graphs represent means (SD).