Table 1.
Forward and reverse primers used for RT-PCR and quantitative RT-PCR amplification.
| Gene | Accession No. | Primers | Product Size |
|---|---|---|---|
| Ascl2 | NM_008554.3 | FWD: 5′-TGGAAGCACACCTTGACTGG-3′ REV: 5′-TGGACGTTTGCACCTTCAC-3′ |
114 |
| Cdx2 | NM_007673.3 | FWD: 5′-CCAGCTCTTTGCCTCTCTGT-3′ REV: 5′-TGCCTCTGGCTCCTGTAGTT-3′ |
175 |
| Eomes | NM_001164789.1 | FWD: 5′-AACATGCAGGGCAATAAGATG-3′ REV: 5′-AGCCTCGGTTGGTATTTGTG-3′ |
172 |
| Esrrb | NM_001159500.1 | FWD: 5′-GGGAGCTTGTGTTCCTCATC-3′ REV: 5′-CTACCAGGCGAGAGTGTTCC-3′ |
200 |
| Gcm1 | NM_008103.3 | FWD: 5′-AACACCAACAACCACAACTCC-3′ REV: 5′-CAGCTTTTCCTCTGCTGCTT-3′ |
151 |
| Id2 | NM_010496.3 | FWD: 5′-CAGCATCCCCCAGAACAA-3′ REV: 5′-TCTGGTGATGCAGGCTGA-3′ |
121 |
| Ovol2 (RT-PCR) | NM_026924.3 | FWD: 5′-TGTTCCTTGGAGTCCCACCT-3′ REV: 5′-ACTGACAACTTTGGGGTGCTT-3′ |
426 |
|
Ovol2 (quantitative RT-PCR) |
NM_026924.3 | FWD: 5′-TTCACCCAGCGGTGTTCCTT-3′ REV: 5′-TGTAGCCGCAATCCTCACAC-3′ |
114 |
| Ovol2 (Genotyping) | NM_026924.3 | FWD: 5′-CATAGCCCATGTGTGGCTGCTG-3′ REV: 5′-GCCGGCCTTAAAACATCCCAC-3′ |
847 |
| Prl3b1 | NM_008865.3 | FWD: 5′-CCAACGTGTGATTGTGGTGT-3′ REV: 5′-TCTTCCGATGTTGTCTGGTG-3′ |
175 |
| Syna | NM_001013751.2 | FWD: 5′-CCCTTGTTCCTCTGCCTACTC-3′ REV: 5′-TCATGGGTGTCTCTGTCCAA-3′ |
175 |
| Synb | NM_173420.3 | FWD: 5′-TGAACACCCCAACTGAGCAA-3′ REV: 5′-GGACGAAACAGAGGACCCAA-3′ |
123 |
| Rn18s | NR_003278.3 | FWD: 5′-GCAATTATTCCCCATGAACG-3′ REV: 5′-GGCCTCACTAAACCATCCAA-3′ |
122 |
| Tpbpa | NM_009411.4 | FWD: 5′-CGGAAGGCTCCAACATAGAA-3′ REV: 5′-TCAAATTCAGGGTCATCAACAA-3′ |
141 |
| Ywhaz | NM_001253805.1 | FWD: 5′-CCCTCTTGGCAGCTAATGG-3′ REV: 5′-GGAGTAAGGGGAAAATGTGGG-3′ |
116 |