Figure 3.

H2A.B.3 co-localises with Miwi and Dicer in chromatoid bodies of rounds spermatids and co-immunoprecipitates with Miwi protein. (A) H2A.B.3 co-immunoprecipitates with Miwi (Cell Signalling cat# 2079S) in non-crosslinked total testis lysates following the procedure described previously [14]. Non-targeting IgG control was used as a negative control during immunoprecipitation. (B,C) Hypotonic spreads of male germ cells [14] showing round spermatids indirectly immunostained with antibodies against mouse H2A.B.3 (red), lectin PNA (green), Miwi (B) (white) or Dicer (C) (white) (abcam cat# 13502) and counterstained with DAPI (blue) to visualize DNA. White arrows show the location of chromatoid bodies.