Table 1.
Primers used for quantitative PCR analyses. CSF2: Colony Stimulating Factor 2; DHCR24: 24-dehydrocholesterol reductase; FDPS: Farnesyl Diphosphate Synthase; HMGCR: 3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMGCS1: 3-Hydroxy-3-Methylglutaryl-CoA Synthase 1; IL6: Interleukin 6; IL8: Interleukin 8; LDLR: Low Density Lipoprotein Receptor; LIPG: Lipase G; MVK: Mevalonate kinase; MSMO1: Methylsterol Monooxygenase 1; NPC1: Niemann-Pick C1 Protein; OSC: oxidosqualene cyclase; SC5D: Sterol-C5-Desaturase; TAP1: Transporter associated with Antigen Processing 1; TRAP1: Tumor necrosis factor Receptor Associated Protein 1.
| Primer ID | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
| ACTIN | CCTCACCCTGAAGTACCCCA | TCGTCCCAGTTGGTGACGAT |
| CSF2 | GTCATCTTGGAGGGACCAAGG | CCATGCCTGTATCAGGGTCAG |
| DHCR24 | TGAAGACAAACCGAGAGGGC | CAGCCAAAGAGGTAGCGGAA |
| FDPS | AGCAGGATTTCGTTCAGCAC | TCCCGGAATGCTACTACCAC |
| HMGCR | TACCATGTCAGGGGTACGTC | CCAGTCCTAATGAAACCTTAGAAG |
| HMGCS1 | TGTCCTTTCGTGGCTCACTC | GCCAGCAAGCTTCTGCATTC |
| IL6 | TGCAATAACCACCCCTGACC | CAATCTGAGGTGCCCATGCT |
| IL8 | CCAGTCTTGTCATTGCCAGC | TGACTGTGGAGTTTTGGCTGT |
| LDLR | GAATTTGGCCAGACACGGT | CACCGTACCCAGCTGATTTT |
| LIPG | CAGGCTGTGGACTCAACGAT | TCGGCTTGTCCTGATTCACC |
| MVK | GCTCAAGTTCCCAGAGATCG | ATGGTGCTGGTTCATGTCAA |
| MSMO1 | GGTTCCGAGGTTGGAACACCT | TTCAAATCTCTGCAGACAGCCT |
| NPC1 | TTGTGGTGTTGGCTTTTGCC | GTTCGCGCTCTGTTCCTTTG |
| OSC | ATGGTGGCCCACTTTTCCTC | CTGCACTGACCGCAGGTA |
| SC5D | TTTGCAGAGCAGTGGCGT | CTGGCCATGTGGCTGGATAC |
| TAP1 | CCTCCTTTCCAAGCTCCTCG | GGCCAGCATATGCCTTCAGT |
| TRAP1 | GACGCACCGCTCAACAT | CACATCAAACATGGACGGTTT |