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. 2020 Apr 9;9(4):923. doi: 10.3390/cells9040923

Figure 3.

Figure 3

PPARδ expression in response to Wnt activation during bovine embryonic development. (a) Relative mRNA expression analysis of PPAR family in response to Wnt stimulation with its agonist 6-Bio (400 nM). n = 5 BLs, experiment performed in triplicate sets. ns; non-significant, ** p < 0.01; indicates significant difference. (b) RT-PCR displaying the PPARδ expression during the time course of bovine embryonic development. (c,d) Immunofluorescence analysis showing the concomitant increase in the PPARδ expression during different stages of bovine pre-implantation development in 6-Bio (400 nM) treated group and control group. Original magnification 100×.