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. 2020 Apr 28;23(5):101113. doi: 10.1016/j.isci.2020.101113

Figure 3.

Figure 3

Allicin Increases the Activity of Brown Adipocytes and Oxygen Consumption In Vitro

(A) Dose-dependent effect of allicin on UCP1 expression in primary brown adipocytes on day 6 of brown adipogenesis.

(B) Thermogenic gene expression in brown adipocytes treated with 50 μM allicin or DMSO.

(C) Immunofluorescence staining of UCP1 and BODIPY in brown adipocytes treated with 50 μM rutin or DMSO at day 6 of brown adipogenesis. Scale bars, 50 μm.

(D–E) The protein levels of UCP1 and OXPHOS in brown adipocytes treated with DMSO or Allicin.

(F) Oxygen consumption rates (OCR) at day 6 of brown adipogenesis with Allicin or DMSO treatment. (n = 5).

(G–J) The OCR-related basal metabolic rate(G), ATP levels(H), maximum oxygen consumption(I), and proton leakage(J). (n = 5).

Values represent means ± SEM. Error bars represent SEM; significant differences compared with vehicle controls are indicated by ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (assessed by Student's t test).