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Effects of 12s-HpETE on the redox state of PTEN in Prx III+/+ and Prx III−/- MEF cells. (A) MEF cells were treated with a mixture of 10 μM 12s-HpETE and Lipofectamine 2000 transfection reagent for the indicated times. The cellular protein extracts were alkylated with 10 mM NEM and subjected to nonreducing or reducing SDS-PAGE, followed by Western blot analysis using antibodies to PTEN or actin. (B) The intensity of oxidized PTEN was quantitated using ImageJ software.
