Fig. 11.

p22phox promotes dexamethasone-induced pulmonary vascular remodeling and pulmonary ROS.

(A–D) Wild type mice (WT) and mice deficient of p22phox due to a point mutation in the Cyba gene (nmf333) were orally supplemented with dexamethasone (Dex, 0.3 mg/kg/day) in the drinking water for 12 weeks. Litter mates drunk normal water (Ctr). Formalin-fixed paraffin embedded (FFPE) lung sections were stained with primary antibodies against (A/B) α-smooth muscle actin (α-SMA) or (C/D) 8-hydroxy-2-deoxyguanosine (8OHdG) and visualized with a secondary antibody conjugated with Alexa Fluor 488. Nuclei were visualized with DAPI. (A/C) Representative stainings are shown. (B) α-SMA-positive small- and medium-sized arterioles (<30 μm) were counted and related to the total number of arterioles of the same diameter in four high power fields per lung section (n = 4; *p < 0.05, vs. WTCtr; ^#p < 0.05 vs. WTDex; (1-β)>0.994). (D) Fluorescence intensity of 60 nuclei was measured in four high power fields per lung section (n = 4; *p < 0.05, vs. WTCtr; ^#p < 0.05 vs. WTDex; (1-β) = 1). Two-tailed Student's t-test was used in all cases.