TABLE 3B.

Population statistics for polymorphic loci and for both polymorphic and fixed loci. Filters applied were –max-het-obs 0 –min-maf 0.05 –r 0.5.

Watersheds	Variant positions			Variant and fixed positions
	N	Private	π	N	Private	Sites	Variant sites	Polymorphic sites	PPL %	π
Pigeon and Little Pigeon Rivers	26	128	0.0035	26	128	309,062	409	7	0.0023	0.00000
Little TN and Hiwassee Rivers	32	168	0.0061	32	168	298,544	405	10	0.0034	0.00001
Ocoee and Coosa Rivers	7	99	0.0006	7	99	250,941	409	1	0.0004	0.00000

Statistics include the total average number of individuals genotyped at each locus (N), the number of alleles unique to each population (private), mean nucleotide diversity (π), the total number of sites for each populations (sites), the number of sites across all populations that are variant (variant sites), the number of sites inside each population that are variant (polymorphic sites) and the percentage of polymorphic sites (PPL %).