FIGURE 3.

Effect of PA (0, 100, 200, and 400 μg mL⁻¹) on C. tropicalis survival after 24 and 48 h of treatment. [A (i)] Image displaying the effect of PA on C. tropicalis survival, grown on SDA agar medium. [A (ii)] CFU of C. tropicalis in the absence and presence of PA determined in logarithmic scale. (B) Confocal microscopic examination of C. tropicalis revealing the efficacy of inducing cell death which was assessed using AO/EtBr staining. (C) SEM analysis of C. tropicalis colony morphology in the absence and presence of PA (100, 200, and 400 μg mL⁻¹) after 24 and 48 h of treatment.