Table 1.

Sequences of primers sets used and targeted genes

Intestinal parasite	Names	Primers (5′ → 3′)	Target region	Reference
Protozoa
Cryptosporidium parvum/hominis	1PSF	AAC TTT AGC TCC AGT TGA GAA AGT ACT C	hsp70 gene	(Garces-Sanchez, Wilderer et al. 2009)
	1PSR	CAT GGC TCT TTA CCG TTA AAG AAT TCC
Blastocystis hominis	BH1	GCT TAT CTG GTT GAT CCT GCC AGT	16 like RNA	(Init, Foead et al. 2007)
	BH2	TGA TCC TTC CGC AGG TTC ACC TAC A
Cystoisospora belli	Ib213F	GGA TAT TCC CTG CAG CAT GT	5,8S rRNA/ITS2	(Taniuchi, Verweij et al. 2011)
	Ib213R	CGG GAC ACA ACT CAA CAC TG
Encephalitozoon intestinalis	Eint214F	CAC CAG GTT GAT TCT GCC TGA C	SSU rRNA	(Verweij, Ten Hove et al. 2007)
	Eint214R	CTA GTT AGG CCA TTA CCC TAA CTA CCA
Enterocytozoon bieneusi	EbITS-89F	TGT GTA GGC GTG AGA GTG TAT CTG	SSU rRNA	(Verweij, Ten Hove et al. 2007)
	EbITS-191R	CAT CCA ACC ATC ACG TAC CAA TC
Dientamoeba fragilis	DFpn_1f	GCC AAG GAA GCA CAC TAT GG	SSU rRNA	(Roser, Nejsum et al. 2013)
	DFpn_364r	GTA AGT TTC GCG CCT GCT
Trichomonas intestinalis	TH3	TGT AAA CGA TGC CGA CAG AG	SSU rRNA	(Crucitti, Abdellati et al. 2004)
	TH5	CAA CAC TGA AGC CAA TGC GAG C
Entamoeba histolytica	EnthF	ATG GCC AAT TCA TTC AAT GA	SSU rRNA	(Suzuki, Kobayashi et al. 2008)
	EnthR	TAC TTA CAT AAA GTC TTC AAA ATG T
Entamoeba dispar	EntdF	GTT AGT TAT CTA ATT TCG ATT AGA AC	SSU rRNA	(Suzuki, Kobayashi et al. 2008)
	EntdR	ACA CCA CTT ACT ATC CCT ACC TA
Entamoeba hartmanni	EhartF	GTG AAG AGA AAG GAT ATC CAA AGT	SSU rRNA	(Suzuki, Kobayashi et al. 2008)
	EhartR	ATA TCA TTT TCA ACT ACG AGC
Helminths
Ascaris lumbricoides	Alum96F	GTA ATA GCA GTC GGC GGT TTC TT	ITS1	(Wiria, Prasetyani et al. 2010)
	Alum183R	GCC CAA CAT GCC ACC TAT TC
Enterobius vermicularis	EVpn_1f	CAA CAC TTG CAC GTC TCT TCA	5S rRNA	(Roser, Nejsum et al. 2013)
	EVpn_195r	ATT GCT CGT TTG CCG ATT AT
Trichuris trichiura	TtF	TTG AAA CGA CTT GCT CAT CAA CTT	18S	(Liu, Gratz et al. 2013)
	TtR	CTG ATT CTC CGT TAA CCG TTG TC
Strongyloides stercoralis	Stro-1530F	GAA TTC CAA GTA AAC GTA AGT CAT TAG C	18S	(Verweij, Canales et al. 2009)
	Stro-1630R	TGC CTC TGG ATA TTG CTC AGT TC
Ancylostoma duodenalis	Ad125F	GAA TGA CAG CAA ACT CGT TGT TG	ITS2	(Verweij, Brienen et al. 2007)
	Ad195R	ATA CTA GCC ACT GCC GAA ACG T
Necator americanus	Na58F	CTG TTT GTC GAA CGG TAC TTG C	ITS2	(Verweij, Brienen et al. 2007)
	Na158R	ATA ACA GCG TGC ACA TGT TGC
Schistosoma mansoni	Smcyt748F	CCC TGC CAA ATG AAG AGA AAA C	Mitochondrial DNA	(ten Hove, Verweij et al. 2008)
	Smcyt847R	TGG GTG TGG AAT TGG TTG AAC
Schistosoma intercalatum	Sh110	TTC CTC CAA CTA CCA TCT TAT CTC	Sh110	(Abbasi, King et al. 2007)
	Sm-SL	AAC CGT CAC GGT TTT ACT CTT GTG
Hymenolepsis sp.	Pr-a	TGG TTT TTT GTG CAT CCT GAG GTT TA	COI	(Muehlenbachs, Bhatnagar et al. 2015)
	Pr-b	AGA AAG AAC GTA ATG AAA ATG AGC AAC