Table 4.
Labeling of hematopoietic stem cells with fluorophore.
| Ref. | Agent | Manufacture | Excitation/ Emission (nm) |
Concentration | Incubation (min) |
Evaluation |
|---|---|---|---|---|---|---|
| Parada-Kusz et al. [23] | CellTrace™ Violet Cell Proliferation Kit | Thermo Fisher Scientific Inc. | 405/450 | 5 µM | 20–30 | NR |
| CellTrace™ CSFE | Thermo Fisher Scientific Inc. | 495/519 | 5 µM | 20–30 | NR | |
| Hamilton et al. [21] | Fluorocein | NR | 495/519 | NR | 10 | NR |
| Staal et al. [24] | PKH26 (#PKH26GL) | Sigma Aldrich | 551/567 | 2 µM | 2 | NR |
| Lopez et al. [22] | GFP* | NA | 475/509 | NA | NA | FCT; PCR |
| Ushiki et al. [25] | Cy5.5 | GE Healthcare UK Ltd., Buckinghamshire, UK | 675/694 | 0.4 mg/mL | 15 | FCT |
| AF750 | Invitrogen, Eugene, OR, USA | 749/775 | 0.1 mg/mL | 15 | FCT |
Abbreviations—Ref.: reference; CFSE: 5-(6)-carboxyfluorescein diacetate succinimidyl Ester; GFP: green fluorescent protein; Cy5.5: cyanine 5.5; AF750: Alexa Fluor 750 carboxylic acid, succinimidyl ester; PCR: Polymerase chain reaction; nm: nanometer; µM: micromolar; mg: milligrams; mL: milliliters; min: min; FCT: flow cytometry technique; NR: not reported; Note—* animals genetically modified that express GFP.