Table 4.
Gene expression comparison between TCGA cohort (Non- and High B4GALNT2 expressers and microarray analyisis of LS174T cells (S2/S11 comparison with Neo).
| Gene Name | Non-B4GALNT2 Expressers | High B4GALNT2 Expressers | Consistency | p-Value | |
|---|---|---|---|---|---|
| Mean ± SD | Mean ± SD | ||||
| Genes Down Up-Regulated in LS174T S2/S11 | CD200 | 171 ± 144 | 250 ± 236 | Yes | ≤0.01 |
| NGFRAP1 | 850 ± 641 | 843 ± 5655 | Yes | N.S. | |
| SKAP1 | 220 ± 176 | 205 ± 192 | No | ||
| SLC4A1 | Not expressed | Not expressed | |||
| Genes Down-Regulated in LS174T S2/S11 | FAM110B | 37 ± 33 | 35 ± 42 | Yes | N.S. |
| ALX1 | 3 ± 13 | 4 ± 8 | No | ||
| F5 | 325 ± 968 | 180 ± 627 | Yes | N.S. | |
| INMT | 132 ± 153 | 118 ± 120 | Yes | N.S. | |
| MYH3 | 41 ± 125 | 16 ± 16 | Yes | ≤0.05 | |
| MBOAT2 | 498 ± 282 | 660 ± 405 | No | ||
| ROR1 | 27 ± 39 | 25 ± 31 | No | ||
| RAI14 | 765 ± 420 | 661 ± 586 | Yes | N.S. | |
| FMO3 | 86 ± 620 | 26 ± 25 | Yes | N.S. | |
| PEG10 | 136 ± 401 | 245 ± 685 | No | ||
| NINL | 168 ± 203 | 119 ± 123 | Yes | ≤0.05 | |
| ARMC4 | 4 ± 13 | 6 ± 8 | No | ||
| MID2 | 146 ± 122 | 150 ± 104 | No | ||
| SOX2 | 107 ± 281 | 32 ± 117 | Yes | ≤0.01 | |
| LGALS2 | 77 ± 141 | 128 ± 210 | No | ||
| NPTX1 | 39 ± 95 | 15 ± 29 | Yes | ≤0.01 | |
| GALC | 644 ± 525 | 663 ± 503 | No | ||
| STARD3NL | 697 ± 255 | 646 ± 253 | Yes | ≤0.1 | |
| ZNF22 | 625 ± 239 | 638 ± 267 | No | ||
| NID1 | 1558 ± 993 | 1544 ± 1496 | Yes | N.S. |
The cohorts of non-expressers (Mean ± SD = 0 ± 0) and of high-expressers (Mean ± SD = 367 ± 69) represent the 15% lower and higher percentiles of the TCGA cohort. The column “Consistency” indicates whether the difference in gene expression of non- or high B4GALNT2 expressers was consistent with that observed by microarray analysis of our LS174T model. Genes showing statistically significant consistent difference are indicated in bold (p ≤ 0.05 Student’s t test for independent samples). N.S. = non-significant.