Figure 3.

The effects of DP agonists on neurite outgrowth. Undifferentiated NSC-34 cells were exposed to various concentrations of BW245C (a DP1 agonist) or 15(R)-15-methyl PGD₂ (a DP2 agonist) for 24 h. (A) Representative phase-contrast microscopy images of NSC-34 cells treated with various concentrations of BW245C (upper panel). Scale bar: 50 μm. Graphs show the percentage of neurite-bearing cells (middle panel) and the average neurite length (lower panel) in each treatment group. (B) Representative phase-contrast microscopy images of NSC-34 cells treated with various concentrations of 15(R)-15-methyl PGD₂ (upper panel). Scale bar: 50 μm. Graphs show the percentage of neurite-bearing cells (middle panel) and the average neurite length (lower panel) in each treatment group. Each value represents the mean ± SEM of four different experiments.