Figure 5.

Conversion of PGD₂ to 15-deoxy-Δ^12,14-prostaglandin J₂ (15d-PGJ₂) under cell-free conditions. Freshly prepared 15 μM PGD₂ was made by diluting a stock solution in cell-free culture medium. Time-dependent concentrations of PGD₂ and 15d-PGJ₂ were measured at 0, 0.5, 1, 2, 4, and 24 h. The left and right axes represent the concentration of PGD₂ (black circles) and 15d-PGJ₂ (white circles), respectively. Each value represents the mean ± SEM of three different experiments. ** p < 0.01, *** p < 0.001 vs. PGD₂ at 0 h. ^# p < 0.05, ^### p < 0.001 vs. 15d-PGJ₂ at 0 h.