Figure 6.
The effect of 15d-PGJ2 on neurite outgrowth. (A) Representative phase-contrast microscopy images of NSC-34 cells treated with various concentrations of 15d-PGJ2. Scale bar: 50 μm. (B) Graphs show the percentage of neurite-bearing cells (left panel) and the average neurite length (right panel) in each treatment group. Each value represents the mean ± SEM of four different experiments. * p < 0.05, *** p < 0.001. (C) Photographs show typical fluorescence images of calcein-AM (green, live cells) and EthD-1 (red, dead cells) double staining in each treatment group. Scale bar: 50 μm. Graphs show the percentage of EthD-1-positive dead cells. Each value represents the mean ± SEM of four different experiments.