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. 2020 Apr 10;9(4):932. doi: 10.3390/cells9040932

Figure 1.

Figure 1

Xanthohumol (Xn) action on colon cancer proliferation and viability. (A) Chemical structure of Xn: 3′-(3,3-dimethylallyl)-2′,4′,4-trihydroxy-6′-methoxychalcone. (B) After treatment of SW620, SW480, and HT29 cells with increasing Xn concentrations (0–50 μM) at 37 °C for 24, 48, and 72 h, the percentage of cell viability was determined by crystal violet assay. Results are expressed as mean percentage of control growth ± SD of three independent experiments with n = 6. (C) After 24 h of culture, colon cancer cells SW620, SW480, and HT29 were treated with medium containing vehicle control (0.1% dimethyl sulfoxide (DMSO); Co) or increasing concentrations of Xn. Treated and control cells were harvested at 0, 24, 48, and 72 h. Cell proliferation was quantified with a hemocytometer. Viable cells were distinguished by trypan blue exclusion. The data are mean ± SD of three independent experiments with n = 6. p values were determined by one-way ANOVA followed by Tukey’s multiple comparison test. * p < 0.05, ** p < 0.01, and *** p < 0.001.