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. 2020 Apr 12;9(4):951. doi: 10.3390/cells9040951

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of PM modulation on heat-induced heat shock response. (A) B16-F10 cells were incubated for 10 min with 10 mM Methyl-β-cyclodextrin (MβCD) at 37 °C followed by 30, 60, or 90 min of heat stress at 42 °C and 3 h recovery at 37 °C; (B) B16-F10 cells were incubated for 2, 5, or 10 min with 10 mM MβCD at 37 °C followed by 1 h heat shock at 42 °C; (C) B16-F10 cells were exposed for 1 h to 50 µg/mL nystatin at 37 °C followed by 1 h heat stress at 42 °C and 3 h recovery at 37 °C; (D) B16-F10 cells were exposed for 1 h to 50 µg/mL nystatin at 37 °C followed by heat stress for 1 h at 42 °C. Bar graphs show quantified band intensities normalized to GAPDH (n = 3), * p < 0.05; ** p < 0.01. CD (X): cells exposed for 2, 5, or 10 min to MβCD. DMSO was treated as a vehicle control for nystatin. A.U.: arbitrary units, HS: heat shock.

Effect of PM modulation on heat-induced heat shock response. (A) B16-F10 cells were incubated for 10 min with 10 mM Methyl-β-cyclodextrin (MβCD) at 37 °C followed by 30, 60, or 90 min of heat stress at 42 °C and 3 h recovery at 37 °C; (B) B16-F10 cells were incubated for 2, 5, or 10 min with 10 mM MβCD at 37 °C followed by 1 h heat shock at 42 °C; (C) B16-F10 cells were exposed for 1 h to 50 µg/mL nystatin at 37 °C followed by 1 h heat stress at 42 °C and 3 h recovery at 37 °C; (D) B16-F10 cells were exposed for 1 h to 50 µg/mL nystatin at 37 °C followed by heat stress for 1 h at 42 °C. Bar graphs show quantified band intensities normalized to GAPDH (n = 3), * p < 0.05; ** p < 0.01. CD (X): cells exposed for 2, 5, or 10 min to MβCD. DMSO was treated as a vehicle control for nystatin. A.U.: arbitrary units, HS: heat shock.