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. 2020 Apr 10;9(4):936. doi: 10.3390/cells9040936

Figure 2.

Figure 2

hnRNP A1 promotes alternative exon 10 skipping of Tau pre-mRNA. (A) (Left) Schematic of Tau1 minigene showing exons with boxes, introns with lines. Vector sequences are shown with arc. The primers used in RT-PCR analysis are shown with arrows. The length of deleted and remained intron are shown. (Middle) RT-PCR monitoring relative exon 10 skipped isoforms within Tau1 minigene in hnRNP A1 or control pcDNA (3.1) expressed cells. Immunoblotting analysis with anti-hnRNP A1 and α-tubulin are shown. (Right) Bar chart of RT-PCR of Tau1 is shown. (B) (Left) Schematic of Tau2 minigene with the length of remained intron 9 and 10. Primers for RT-PCR are shown with arrows. (Middle) RT-PCR analysis of relative exon 10 skipped isoforms within Tau2 minigene in hnRNP A1 or control pcDNA (3.1) expressed cells. (Right) Bar chart of RT-PCR of Tau2 splicing is shown. (C) RT-PCR monitoring of alternative splicing of exon 10 in cells transfected with different concentration hnRNP A1. The hnRNP A1 concentrations are shown on the top of lanes. GAPDH is used as a loading control.