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. 2020 Apr 10;9(4):928. doi: 10.3390/cells9040928

Figure 1.

Figure 1

S1PR expression and signaling in EC. (A) qPCR analysis of S1PR expression in EA.hy926 and HUVEC. Dara are means ± SEM, n = 3. (B) Flow Cytometric analysis cell surface expression of S1PR1 on EC before and after treatment with 1 µM FTY720 overnight. means ± SEM, n = 3. (C) Intracellular calcium responses in EA.hy926 and HUVEC upon stimulation with 100 nM S1P. Data were normalized to the response of 10 µM ATP. Means ± SEM, n = 3. (D) Resistance following treatment with 1 µM S1P, normalized resistance values were taken at the time of the established maximum resistance after S1P treatment divided by resistance of carrier-treated control cells at the same time and are means ± SEM, n = 3, ** p <0.01, determined by two-sided Student’s t-test. Line plots represent one experiment out of three with black arrows indicating the addition of S1P or vehicle at the corresponding time. (E) Difference in initial non-stimulated resistance of EA.hy926 and HUVEC in ECIS measurements 60 h after seeding, means ± SEM, n = 3, * p < 0.05, determined by a two-sided Student’s t-test. Line plot represents one experiment out of three.