Figure 2.

Distribution of PBE motifs in PUM1 and PUM2 targets. (A) Content of PUM1- and PUM2-regulated mRNAs in the TCam-2 mRNA transcriptome. (B) Content of mRNAs containing at least one PBE in the whole sequence, at three steps of selection: (1) RIP-Seq approach; (2) PUM1 or PUM2 siRNA knockdown; and (3) combined RIP-Seq/RNA-Seq upon PUMs knockdown (regulated). (C) PBE distribution in particular regions of mRNAs whose level was changed upon PUM1 (repressed—first, activated—second from the left) and PUM2 (repressed—third, activated—fourth from the left) siRNA knockdown, respectively. (D) PBE distribution in particular regions of regulated mRNAs by PUM1 (repressed—first, activated—second from the left) and PUM2 (repressed—third, activated—fourth from the left), respectively.