Figure 6.

Polarised transport and transcytosis in hiPSC-derived BMECs. (A) Permeability of transported substrates across hiPSC-derived BMECs (Cl1) in presence of different inhibitors. Coefficients in the A-B and in the B-A directions are reported as mean ± sd of at least three biological replicates along with respective efflux ratio. Statistical differences between both sides is reported as the p-value; ns = not significant. The statistical analysis for the difference of permeability for the compound alone or in the presence of an inhibitor is indicated by asterisks: ***for p < 0.001, ** for p < 0.01 and * for p < 0.05. (B) hiPSC-derived BMECs support transcytosis of the anti-transferrin antibody MEM189. Amount of the antibody measured at 37 and 4 °C in the basal compartment after 2 and 4 h is the mean ± sd of duplicate determination of biological triplicates. (C) Permeability coefficient and percentage of transported dose of the anti-transferrin antibody MEM-189 in the experimental conditions as reported in B.