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. 2020 May 8;11:618. doi: 10.3389/fphar.2020.00618

Figure 2.

Figure 2

BRD4 inhibition prevents the activation of cell death pathway and downregulation of proinflammatory gene expression in vitro. (A) ATP levels were measured by luminescence in isolated pancreatic acinar cells. Data were normalized to untreated control as 100% (n = 5). (B) The percentage of total cellular lactate dehydrogenase (LDH) released into the extracellular medium in isolated pancreatic acinar cells stimulated with CCK (200 nmol·L-1) in the absence or presence of JQ1 (500 nmol·L-1) (n = 5). (C) PI uptake induced by CCK (200 nmol·L-1) with or without JQ1 (500 nmol·L-1) in isolated pancreatic acinar cells (n = 5). (D) mRNA levels of Tnf, Il1b, and Il6 in CCK-stimulated pancreatic acinar cells with or without JQ1 (n = 3). Data represent the mean values ± SEM. Statistical analysis was performed by Student’s un-paired, two-tailed t-test between two groups, *P < 0.05, compared to the control; #P < 0.05, compared to CCK-stimulated group.