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. 2020 Apr 11;9(4):943. doi: 10.3390/cells9040943

Figure 1.

Figure 1

Oxidative stress induces apoptosis and autophagosome formation in B4G12 and ARPE-19 cells. The HCEC cell line (B4G12) and the RPE cell line (ARPE-19) were treated with paraquat (an oxidative stress inducer, 2 mM for ARPE-19, 0.2 mM for B4G12) for five days. Cell morphology was observed using phase-contrast microscopy. TUNEL assay and immunofluorescence of LC3 were performed to examine apoptosis and autophagosome formation (green color). Nuclei were counterstained with Hoechst 33342 (blue color). The scale bars represent 100 µm.