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. 2020 Apr 10;9(4):938. doi: 10.3390/cells9040938

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of IL-26 on mRNA expression in M1 and M2 macrophage differentiation. RAW264.7 cells were treated with IFN-γ (20 ng/mL), IL-4 (20 ng/mL), or LPS (10 ng/mL) in the presence or absence of IL-26 for 3, 6, and 12 h. Total RNA was isolated, and 1 μg of total RNA was used to transcribe cDNA. cDNA was used as a template for PCR with mouse-specific primers. M1 macrophage gene markers (A) CD80, (B) TNF-α, or (C) iNOS, and M2 macrophage gene markers (D) IL-10 or (E) CD206 were detected by QPCR. Results are the means ± SD of three independent experiments. (* p < 0.05, ** p < 0.01, *** p < 0.001).

Effects of IL-26 on mRNA expression in M1 and M2 macrophage differentiation. RAW264.7 cells were treated with IFN-γ (20 ng/mL), IL-4 (20 ng/mL), or LPS (10 ng/mL) in the presence or absence of IL-26 for 3, 6, and 12 h. Total RNA was isolated, and 1 μg of total RNA was used to transcribe cDNA. cDNA was used as a template for PCR with mouse-specific primers. M1 macrophage gene markers (A) CD80, (B) TNF-α, or (C) iNOS, and M2 macrophage gene markers (D) IL-10 or (E) CD206 were detected by QPCR. Results are the means ± SD of three independent experiments. (* p < 0.05, ** p < 0.01, *** p < 0.001).