Figure 1.
Polymer block in ELISA of CTB binding to triLex and GM1. (A and C) Plates were coated with triLex-HSA and probed with CTB preincubated with the different polymers. (B and D) Plates were coated with GM1-HSA and probed with CTB preincubated with the different polymers. In (A) and (B), all polymers used displayed 100 sugars. In (C) and (D), the length of the copolymer varies with the number of displayed sugars. Curve fits were made using a three-parameter fit to eq 1. Dotted lines indicate unblocked CTB binding. Graphs show a representative experiment out of three independent experiments, and error bars are the SD of intra-assay duplicates or quadruplicates.