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. 2020 Mar 5;6(5):1192–1203. doi: 10.1021/acsinfecdis.0c00009

Figure 3.

Figure 3

Flow cytometry evaluation of glycopolymers’ capacity to block CTB and LTB binding in human SI enterocytes. Cells were isolated from human SI and stained for common cell markers and with CTB to analyze the polymer block by flow cytometry. Full gating can be seen in Figure S6. Panel (A) shows representative histograms of CTB binding to EpCAM+ cells with or without a polymer block. Panels (B) and (C) show graphs of gMFI for CTB binding after long polymer (B) and short polymer (C) blocks (n = 5–10 donors). (D) Representative (out of five donors) histogram of LTB binding to EpCAM+ cells with or without a polymer block. (E) Representative (out of five donors) histogram of CTB and LTB binding to EpCAM+ cells. The values are normalized to the % of unblocked CTB gMFI. Error bars represent the SD.