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. 2020 May 14;17:156. doi: 10.1186/s12974-020-01814-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Expression of IL-20, IL-20R1, and IL-20R2 in spinal cord tissues after SCI. The transverse sections around the interface between gray and white matters of the spinal cord obtained from SCI rats (N = 5). a Double immunofluorescence staining of IL-20 (green) with markers for specific neural cell types (red) including NeuN (neurons), Olig2 (oligodendrocytes), GFAP (astrocytes), and Iba1 (microglia). Nuclei were counterstained with DAPI (blue). Co-localization of IL-20 with each cellular marker appears yellow in the merged image. Scale bars = 500 μm. b, c Double immunofluorescence staining of IL-20R1 (green) or IL-20R2 (green) with markers for specific neural cell types (red) as described above. Co-localization of IL-20R1 or IL-20R2 with each cellular marker appears yellow in the merged image. Scale bars = 500 μm. NeuN, neuronal nuclei; Olig2, oligodendrocyte transcription factor 2; GFAP, glial fibrillary acidic protein; Iba1, ionized calcium-binding adapter molecule 1. Data from one representative experiment of three independent experiments is shown